Effects Of Gentamicin Loaded Pcl Nanofibers To Cell Viability And Release Rate Of Plasmid Dna
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Tarih
2020
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Polycaprolactone (PCL) nanofiber with constant plasmid DNA addition and with different concentration of gentamicin designed and studied for their effect on cell viability and release rate. PCL nanofibers were fabricated using electrospinning method with plasmid DNA and gentamicin addition. The plasmid DNA used in PCL nanofiber were extracted from E. coli. The scanning electron microscopy (SEM) images show that the nano-scale fiber structures have an average diameter of 113.9 nm. UV microplate reader confirmed the existence of plasmid DNA in the PCL nanofibers. Elisa reader study showed the addition of gentamicin in the fibers. Cell viability tests indicated that PCL nanofibers with 10% gentamicin on a fibroblast cell showed high cell viability, which is related to surface areas and pore size of the electrospun fibers besides to the interaction among gentamicin, plasmid DNA and electrospun fiber matrix.
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EskiÅŸehir Teknik Ãœniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler
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Cilt
8
Sayı
2
