Yazar "Ceylan, Muhammet" seçeneğine göre listele
Listeleniyor 1 - 12 / 12
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Biodegradable Polycaprolactone Fibers with Silica Aerogel and Nanosilver Particles Produce a Coagulation Effect(MDPI, 2023) Ayvazoğlu, Büşra Şengel; Ceylan, Muhammet; Turan, Aybüke A. Isbir; Yılmaz, Elif BurcuPoly-?-caprolactone (PCL) is a biodegradable aliphatic polyester that can be used in the field of biomaterials. Electrospinning is the name given to the process of producing micro and nanoscale fibers using electrostatically charged polymeric solutions under certain conditions. Almost all synthetic and naturally occurring polymers can undergo electrospinning using suitable solvents or mixtures prepared in certain proportions. In this study, silica aerogels were obtained by the sol-gel method. PCL-silica aerogel fibers were synthesized by adding 0.5, 1, 2, and 4% ratios in the PCL solution. Blood contact analysis was performed on the produced fibers with UV-VIS. According to the results obtained, 0.5, 1, 2, and 4% nano-silver were added to the fiber-containing 4% aerogel. Then, SEM-EDS and FTIR analyses were performed on all fibers produced. Antimicrobial tests were performed on fibers containing nano-silver. As a result, high-performance blood coagulation fibers were developed using PCL with aerogel, and an antimicrobial effect was achieved with nano-silver particles. It is thought that the designed surface will be preferred in wound dressing and biomaterial in tissue engineering, as it provides a high amount of cell adhesion with a small amount of blood and contains antimicrobial properties.Öğe Effects Of Gentamicin Loaded Pcl Nanofibers To Cell Viability And Release Rate Of Plasmid Dna(2020) Ceylan, Muhammet; Yang, Shang You; Asmatulu, RamazanPolycaprolactone (PCL) nanofiber with constant plasmid DNA addition and with different concentration of gentamicin designed and studied for their effect on cell viability and release rate. PCL nanofibers were fabricated using electrospinning method with plasmid DNA and gentamicin addition. The plasmid DNA used in PCL nanofiber were extracted from E. coli. The scanning electron microscopy (SEM) images show that the nano-scale fiber structures have an average diameter of 113.9 nm. UV microplate reader confirmed the existence of plasmid DNA in the PCL nanofibers. Elisa reader study showed the addition of gentamicin in the fibers. Cell viability tests indicated that PCL nanofibers with 10% gentamicin on a fibroblast cell showed high cell viability, which is related to surface areas and pore size of the electrospun fibers besides to the interaction among gentamicin, plasmid DNA and electrospun fiber matrix.Öğe Effects of gentamicin-loaded PCL nanofibers on growth of Gram positive and Gram negative bacteria(Blue Pen, 2017) Ceylan, Muhammet; Yang, Shang-You; Asmatulu, RamazanPoly-?-caprolactones (PCLs) incorporated with gentamicin of different concentrations (0, 2.5, 5 and 10 wt.%) were electrospun under various conditions, and the resultant nanofibers of different thicknesses (1, 2 and 4 layers) were used against the growth of Gram-negative and Gram-positive bacteria, such as Escherichia coli, Salmonella sp. and Staphylococcus epidermidis. PCL polymer was selected mainly because of its biodegradable aliphatic polyester characteristics and also, it plays a critical role in tissue engineering and pharmaceuticals. Scanning electron microscopy (SEM) images showed that the resultant fibers were in the range of 50 to 200 nm with an average diameter of 100 nm. Bacterial test results revealed that the gentamicin molecules in the nanofibers were gradually released from the PCL nanofibers during the in vitro tests and prevented bacterial growth at different inhibition zones and kinetics. Overall, this work provides a detailed explanation of how to improve the antibacterial properties of new drug delivery systems for many biomedical fields, such as scaffolding; drug, DNA, and protein delivery; and wound healing.Öğe Experimental investigation on mechanical properties of Multi Wall Carbon Nanotubes (MWCNT) reinforced aluminium metal matrix composites(KOREAN ASSOC CRYSTAL GROWTH, INC, 2020) Topcu, Ismail; Ceylan, Muhammet; Yilmaz, Elif BurcuThe aim of this research is to obtain better micrographs and to perform the mechanical characterization of Multi Wall Carbon Nanotubes (MWCNT) consolidated aluminum matrix composite (AMC). This research is focused on the mechanical behavior and microstructure characteristics in MWCNT consolidated aluminum matrix composites. The composites of MWCNT/Al were successfully fabricated using powder metallurgy (PM) mechanical alloying (MA) sintering. X-ray diffraction (XRD) and Scanning electron microscopy (SEM) micrographs were utilized in order to determine the porosity, density and hardness characteristics to observe if they are affected or not concerning the performance of composites in different reinforced % MWCNT rate conditions. Along with SEM and XRD studies, this study details the homogeneous distribution of nanotubes which showed no evidence of decomposition; this was supported by a successive increase in hardness and abrasion resistance. This increase in abrasion resistance and hardness can be accredited to the further activation of the slip system due to the presence of MWCNTs. Based on the conclusions of this study, the authors determined that the mechanical alloying sintering method provides promising results for the mechanical alloying fabrication of MWCNT/Al composites, especially due to the different % rate reinforced up capabilities.Öğe Fabrication of polycaprolactone - silica aerogel nanofibers via electrospinning method(TRANS TECH PUBLICATIONS LTD, 2022) Ceylan, Muhammet; Yılmaz, Elif BurcuThe field of nanotechnology has seen rapid advancements over the last decade. Nanofiber production through the method of electrospinning is one of the attraction points in this area. The nanofibers, prepared with nano-sized additives, particularly with polymer, have an extensive range of usages. This study utilizes silica aerogels obtained by the sol-gel method due to their low density of 700-800 gr/m2. Polycaprolactone (PCL)-Silica Aerogel Nanofibers were attained by adding 0.5%,1%, 2%and 4% of previously produced aerogels to the nanofibers formed by electrospinning. This paper correspondingly examined the differences between AC-CL and MET-CL solvent groups being utilized during the preparation of the solutions. In addition to this examination, series of material tests were conducted, such as tensile test, SEM, FTIR, DTA/TG, and BET. Overall, the resultant nanofibers with a property of high surface area can be utilized in the design of materials applied to many areas, including solar devices, solar pools, sensors, and capacitors.Öğe Poly-?-caprolactone electrospun nanofiber mesh as a gene delivery tool(Aims Press, 2016) Jiang, Jianhao; Ceylan, Muhammet; Zheng, Yi; Yao, Li; Asmatulu, Ramazan; Yang, Shang-YouPoly-?-caprolactone (PCL) is a biodegradable aliphatic polyester which plays critical roles in tissue engineering, such as scaffolds, drug and protein delivery vehicles. PCL nanofiber meshes fabricated by electrospinning technology have been widely used in recent decade. The objective of this study intends to develop a gene-tethering PCL-nanofiber mesh that can be used as a wrapping material during surgical removal of primary bone tumors, and as a gene delivery tool to provide therapeutic means for tumor recurrence. Non-viral plasmid vector encoding green fluorescent protein (eGFP) was incorporated into PCL nanofibers by electron-spinning technique to form multilayer nano-meshes. Our data demonstrated that PCL nanofiber mesh possessed benign biocompatibility in vitro. More importantly, pCMVb-GFP plasmid-linked electrospun nanofiber mesh successfully released the GFP marker gene and incorporated into the co-cultured fibroblast cells, and consequently expressed the transgene product at transcriptional and translational levels. Further investigation is warranted to characterize the therapeutic influence and long-term safety issue of the PCL nanofiber mesh as a gene delivery tool and therapeutic device in orthopedic oncologyÖğe Reduced toxicity of protein/magnetic targeted drug delivery system for improved skin cancer treatment in mice model(Elsevier, 2021) Ceylan, Muhammet; Misak, Heath E.; Strong, Nora; Yang, Shang-youSkin cancer is one of the most common types of cancers. Although majority of skin cancers can be treated effectively if they are diagnosed at an early stage, delayed treatment for some types, such as melanoma, are life threatening. This current study evaluated the therapeutic effects of a recently developed targeted drug delivery system (DDS) to treat an experimental skin squamous cell carcinoma (SCC) in a mouse model. This DDS can release drugs into the body at a designated rate and location. The system consists of biodegradable polymer microspheres of encapsulated magnetic nanoparticles, human albumin, and 5-fluorouracil (5-FU). While the magnetic forces keep the nanocomposite spheres in the targeted cancer area, the protein promotes increased absorption of the DDS near cancer cells to release the therapeutic agent. While tumor sizes were significantly decreased and cancer tissue underwent dramatic necrosis, no remote organ and lymph node metastasis were observed. The tissue specimens collected from vital organs (lungs, liver, kidneys, and lymph nodes adjcent to the cancer sites) were histologically examined and did not showobvious damages and adverse tissue responses after the DDS injections. Overall, this study confirmed that this DDS is successful in treating cancer without toxic effects and may be a possible alternative to traditional therapies.Öğe Studying the electrochemical behaviors of anodized metallic implants for improved corrosion resistance(İstanbul Ticaret Üniversitesi, 2022) Murad, Md. Shafinur; Usta, Aybala; Asmatulu, Ramazan; Ceylan, MuhammetA study about long-term corrosion behavior of anodized and non-anodized Ti6Al4V and MgAZ31B biomaterials was conducted under controlled conditions. By applying 20V DC potential, MgAZ31B alloys was anodized in phosphoric acid and potassium hydroxide while Ti6Al4V alloys was anodized in phosphoric acid and oxalic acid. Long-term experiments were carried out by immersing them in deionized (DI) water, 3% NaCl and phosphate-buffered saline (PBS) solutions. The corrosion rate and pattern were measured by electrochemical analysis. Also, as a result of anodization, the natural oxide layer was observed on the material surface, thus the corrosion rate is reduced and the life of the biomaterial has been improved.Öğe Synthesis and characterization of platinum nanocomposites(Eskişehir Teknik Üniversitesi, 2018) Ceylan, Muhammet; Tepekıran, Beyza NurThis work related to fabrication of platinum nano particles, reveals considerably outstanding catalytic and electrical properties and preparation of Pt- PS nano composite. For this study Pt nano particles were synthesized by wet chemical method. Pt particles with 0.25%, 0.5%, 1% and 2% concentration amounts were added into the polystyrene solution, acquired by recycled PS. Three different composites which possess a distinct percentage of platinum were produced. Fibers were fabricated by electro spinning method afterward. Pt particles which were produced by wet chemical method and fibers were analyzed by SEM-EDS. There were various test and measurement methods performed to determine the characteristics of the platinum nano composite.Öğe Synthesis And Characterization Of Polycaprolactone Nanocomposite Fiber With Titanium Dioxide Additives(2020) Yılmaz, Elif Burcu; Ceylan, MuhammetNanotechnology can control the atomic, molecular, and supramolecular levels of matter. As the size of a particle goes down to nano-size, materials exhibits good characteristics in terms of electronics, optic, thermal, magnetic and fotocatalysis properties. The nano composite fibers comprise of finely dispersed flakes of the second phase inorganic material with nylon or polyester type fiber forming polymers. Nano-fiber based materials obtained by the method of electrospining in the interest of the outstanding features has increased due to the common application areas of filtration, composites, medicine. In this research, we produced nano composites at 0.50 1, 2, and 4 % ratio using polycaprolactone (PCL) and titanium dioxide (TiO2). Energy Dispersive X-Ray Spectroscopy (EDS) and Scanning Electron Microscopy (SEM) were used to analyze titanium dioxide nano fibers.Öğe Synthesis and evaluation of electrospun PCL-plasmid DNA nanofibers for post cancer treatments(Elsevier Ltd, 2022) Ceylan, Muhammet; Asmatulu, Ramazan; Jiang, Jianhao; Usta, Aybala; Jia, Tanghong; Yao, Li; Yang, Shang-youPoly-?-caprolactone (PCL) incorporated with plasmid DNA was electrospun, and the resultant nanofibers were used to observe DNA release from the nanofibers. The plasmid DNA enhanced green fluorescent protein (EGFP) with cytomegalovirus (CMV) promoter (PCMVb-GFP) was amplified with E. coli. PCL was chosen because it is biodegradable aliphatic polyester, which plays a critical role in tissue engineering, such as scaffolding, drug, DNA, gene and protein delivery vehicles. Some of the physical and biological properties of the nanofibers were determined using different methods. Scanning electron microscopy (SEM) micrographs showed that nanofibers have an average diameter of about 100 nm. Cytotoxicity tests showed that cell viability for 1 day, 4 days, and 7 days of the tests were above 80 %. These data demonstrated that PCL-plasmid DNA nanofibers have no cytotoxicity and showed benign biocompatibility for biomedical applications. PCMVb-GFP plasmid-linked electrospun nanofibers continuously released double-stranded DNA for at least seven days. For the first 15 min, there was a burst release of about 1.8 ng/ml. For the following hours and days, the release was about to be the same (release of 0.575 ng/ml). Therefore, PCL nanofibers may be an ideal candidate for various biomedical applications such as cancer treatment, scaffolding, tissue engineering, and protein delivery vehicles.Öğe Wear behavior of irregular shape Ti6Al4V powder reinforced with carbon nanotubes(KOREAN ASSOC CRYSTAL GROWTH, INC, 2020) Ceylan, Muhammet; Topcu, IsmailThe purpose of this study was to investigate the sintering behavior, microstructural evolution, and the effect on wear resistance of carbon nanotubes (CNT) with the addition of a mechanical alloy (Ti6Al4V) and sintered irregular Ti64 powder. The mechanical alloy powders utilized in this study were produced through the process of CIP (Cold Isostatic Press) in order to produce samples by compress on under various pressures within a 300 MPa floating molded press. The samples were sintered at a high vacuum (10-5 mbar) for 60 minutes at a temperature of 1275 degrees C. After sintering, the materials were characterized using an optic microscope (OM), scanning electron microscopy (SEM) and EDX (Energy-dispersive X-ray spectroscopy) to determine whether the materials had wear resistance, density measurement, etc. The Carbon Nanotube wear and friction behavior were investigated under various conditions using a pin wear tester on a disc followed by a scanning electron microscopy (SEM) analysis. The objective of this study was to evaluate the density, metallographic properties and hardness of Ti64 samples supplemented with different CNT ratios as a function of sintering temperature. Theoretical density and micro-hardness of mechanical alloyed and sintered irregular Ti64 powders changed with the additions to CNT under increased sintering temperatures.
