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Öğe Effects Of Gentamicin Loaded Pcl Nanofibers To Cell Viability And Release Rate Of Plasmid Dna(2020) Ceylan, Muhammet; Yang, Shang You; Asmatulu, RamazanPolycaprolactone (PCL) nanofiber with constant plasmid DNA addition and with different concentration of gentamicin designed and studied for their effect on cell viability and release rate. PCL nanofibers were fabricated using electrospinning method with plasmid DNA and gentamicin addition. The plasmid DNA used in PCL nanofiber were extracted from E. coli. The scanning electron microscopy (SEM) images show that the nano-scale fiber structures have an average diameter of 113.9 nm. UV microplate reader confirmed the existence of plasmid DNA in the PCL nanofibers. Elisa reader study showed the addition of gentamicin in the fibers. Cell viability tests indicated that PCL nanofibers with 10% gentamicin on a fibroblast cell showed high cell viability, which is related to surface areas and pore size of the electrospun fibers besides to the interaction among gentamicin, plasmid DNA and electrospun fiber matrix.Öğe Effects of gentamicin-loaded PCL nanofibers on growth of Gram positive and Gram negative bacteria(Blue Pen, 2017) Ceylan, Muhammet; Yang, Shang-You; Asmatulu, RamazanPoly-?-caprolactones (PCLs) incorporated with gentamicin of different concentrations (0, 2.5, 5 and 10 wt.%) were electrospun under various conditions, and the resultant nanofibers of different thicknesses (1, 2 and 4 layers) were used against the growth of Gram-negative and Gram-positive bacteria, such as Escherichia coli, Salmonella sp. and Staphylococcus epidermidis. PCL polymer was selected mainly because of its biodegradable aliphatic polyester characteristics and also, it plays a critical role in tissue engineering and pharmaceuticals. Scanning electron microscopy (SEM) images showed that the resultant fibers were in the range of 50 to 200 nm with an average diameter of 100 nm. Bacterial test results revealed that the gentamicin molecules in the nanofibers were gradually released from the PCL nanofibers during the in vitro tests and prevented bacterial growth at different inhibition zones and kinetics. Overall, this work provides a detailed explanation of how to improve the antibacterial properties of new drug delivery systems for many biomedical fields, such as scaffolding; drug, DNA, and protein delivery; and wound healing.Öğe Poly-?-caprolactone electrospun nanofiber mesh as a gene delivery tool(Aims Press, 2016) Jiang, Jianhao; Ceylan, Muhammet; Zheng, Yi; Yao, Li; Asmatulu, Ramazan; Yang, Shang-YouPoly-?-caprolactone (PCL) is a biodegradable aliphatic polyester which plays critical roles in tissue engineering, such as scaffolds, drug and protein delivery vehicles. PCL nanofiber meshes fabricated by electrospinning technology have been widely used in recent decade. The objective of this study intends to develop a gene-tethering PCL-nanofiber mesh that can be used as a wrapping material during surgical removal of primary bone tumors, and as a gene delivery tool to provide therapeutic means for tumor recurrence. Non-viral plasmid vector encoding green fluorescent protein (eGFP) was incorporated into PCL nanofibers by electron-spinning technique to form multilayer nano-meshes. Our data demonstrated that PCL nanofiber mesh possessed benign biocompatibility in vitro. More importantly, pCMVb-GFP plasmid-linked electrospun nanofiber mesh successfully released the GFP marker gene and incorporated into the co-cultured fibroblast cells, and consequently expressed the transgene product at transcriptional and translational levels. Further investigation is warranted to characterize the therapeutic influence and long-term safety issue of the PCL nanofiber mesh as a gene delivery tool and therapeutic device in orthopedic oncologyÖğe Studying the electrochemical behaviors of anodized metallic implants for improved corrosion resistance(İstanbul Ticaret Üniversitesi, 2022) Murad, Md. Shafinur; Usta, Aybala; Asmatulu, Ramazan; Ceylan, MuhammetA study about long-term corrosion behavior of anodized and non-anodized Ti6Al4V and MgAZ31B biomaterials was conducted under controlled conditions. By applying 20V DC potential, MgAZ31B alloys was anodized in phosphoric acid and potassium hydroxide while Ti6Al4V alloys was anodized in phosphoric acid and oxalic acid. Long-term experiments were carried out by immersing them in deionized (DI) water, 3% NaCl and phosphate-buffered saline (PBS) solutions. The corrosion rate and pattern were measured by electrochemical analysis. Also, as a result of anodization, the natural oxide layer was observed on the material surface, thus the corrosion rate is reduced and the life of the biomaterial has been improved.Öğe Synthesis and evaluation of electrospun PCL-plasmid DNA nanofibers for post cancer treatments(Elsevier Ltd, 2022) Ceylan, Muhammet; Asmatulu, Ramazan; Jiang, Jianhao; Usta, Aybala; Jia, Tanghong; Yao, Li; Yang, Shang-youPoly-?-caprolactone (PCL) incorporated with plasmid DNA was electrospun, and the resultant nanofibers were used to observe DNA release from the nanofibers. The plasmid DNA enhanced green fluorescent protein (EGFP) with cytomegalovirus (CMV) promoter (PCMVb-GFP) was amplified with E. coli. PCL was chosen because it is biodegradable aliphatic polyester, which plays a critical role in tissue engineering, such as scaffolding, drug, DNA, gene and protein delivery vehicles. Some of the physical and biological properties of the nanofibers were determined using different methods. Scanning electron microscopy (SEM) micrographs showed that nanofibers have an average diameter of about 100 nm. Cytotoxicity tests showed that cell viability for 1 day, 4 days, and 7 days of the tests were above 80 %. These data demonstrated that PCL-plasmid DNA nanofibers have no cytotoxicity and showed benign biocompatibility for biomedical applications. PCMVb-GFP plasmid-linked electrospun nanofibers continuously released double-stranded DNA for at least seven days. For the first 15 min, there was a burst release of about 1.8 ng/ml. For the following hours and days, the release was about to be the same (release of 0.575 ng/ml). Therefore, PCL nanofibers may be an ideal candidate for various biomedical applications such as cancer treatment, scaffolding, tissue engineering, and protein delivery vehicles.
