Poly-?-caprolactone electrospun nanofiber mesh as a gene delivery tool

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dc.authorid0000-0001-6933-2917en_US
dc.contributor.authorJiang, Jianhao
dc.contributor.authorCeylan, Muhammet
dc.contributor.authorZheng, Yi
dc.contributor.authorYao, Li
dc.contributor.authorAsmatulu, Ramazan
dc.contributor.authorYang, Shang-You
dc.date.accessioned2019-08-20T08:03:35Z
dc.date.available2019-08-20T08:03:35Z
dc.date.issued2016en_US
dc.departmentFakülteler, Mühendislik Fakültesien_US
dc.description.abstractPoly-?-caprolactone (PCL) is a biodegradable aliphatic polyester which plays critical roles in tissue engineering, such as scaffolds, drug and protein delivery vehicles. PCL nanofiber meshes fabricated by electrospinning technology have been widely used in recent decade. The objective of this study intends to develop a gene-tethering PCL-nanofiber mesh that can be used as a wrapping material during surgical removal of primary bone tumors, and as a gene delivery tool to provide therapeutic means for tumor recurrence. Non-viral plasmid vector encoding green fluorescent protein (eGFP) was incorporated into PCL nanofibers by electron-spinning technique to form multilayer nano-meshes. Our data demonstrated that PCL nanofiber mesh possessed benign biocompatibility in vitro. More importantly, pCMVb-GFP plasmid-linked electrospun nanofiber mesh successfully released the GFP marker gene and incorporated into the co-cultured fibroblast cells, and consequently expressed the transgene product at transcriptional and translational levels. Further investigation is warranted to characterize the therapeutic influence and long-term safety issue of the PCL nanofiber mesh as a gene delivery tool and therapeutic device in orthopedic oncologyen_US
dc.identifier.doi10.3934/bioeng.2016.4.528en_US
dc.identifier.endpage537en_US
dc.identifier.issue4en_US
dc.identifier.startpage528en_US
dc.identifier.urihttps://hdl.handle.net/11467/2879
dc.identifier.urihttps://doi.org/10.3934/bioeng.2016.4.528
dc.identifier.volume3en_US
dc.identifier.wosWOS:000396609100008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAims Pressen_US
dc.relation.ispartofBioengineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectElectrospun Nanofiberen_US
dc.subjectEGFPen_US
dc.subjectPCLen_US
dc.subjectTissue Engineeringen_US
dc.subjectGene Deliveryen_US
dc.titlePoly-?-caprolactone electrospun nanofiber mesh as a gene delivery toolen_US
dc.typeArticleen_US

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