Synthesis and evaluation of electrospun PCL-plasmid DNA nanofibers for post cancer treatments

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dc.contributor.authorCeylan, Muhammet
dc.contributor.authorAsmatulu, Ramazan
dc.contributor.authorJiang, Jianhao
dc.contributor.authorUsta, Aybala
dc.contributor.authorJia, Tanghong
dc.contributor.authorYao, Li
dc.contributor.authorYang, Shang-you
dc.date.accessioned2023-01-20T11:44:51Z
dc.date.available2023-01-20T11:44:51Z
dc.date.issued2022en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Mekatronik Mühendisliği Bölümüen_US
dc.description.abstractPoly-?-caprolactone (PCL) incorporated with plasmid DNA was electrospun, and the resultant nanofibers were used to observe DNA release from the nanofibers. The plasmid DNA enhanced green fluorescent protein (EGFP) with cytomegalovirus (CMV) promoter (PCMVb-GFP) was amplified with E. coli. PCL was chosen because it is biodegradable aliphatic polyester, which plays a critical role in tissue engineering, such as scaffolding, drug, DNA, gene and protein delivery vehicles. Some of the physical and biological properties of the nanofibers were determined using different methods. Scanning electron microscopy (SEM) micrographs showed that nanofibers have an average diameter of about 100 nm. Cytotoxicity tests showed that cell viability for 1 day, 4 days, and 7 days of the tests were above 80 %. These data demonstrated that PCL-plasmid DNA nanofibers have no cytotoxicity and showed benign biocompatibility for biomedical applications. PCMVb-GFP plasmid-linked electrospun nanofibers continuously released double-stranded DNA for at least seven days. For the first 15 min, there was a burst release of about 1.8 ng/ml. For the following hours and days, the release was about to be the same (release of 0.575 ng/ml). Therefore, PCL nanofibers may be an ideal candidate for various biomedical applications such as cancer treatment, scaffolding, tissue engineering, and protein delivery vehicles.en_US
dc.identifier.doi10.1016/j.matpr.2022.07.447en_US
dc.identifier.endpage63en_US
dc.identifier.scopus2-s2.0-85136763533en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage58en_US
dc.identifier.urihttps://hdl.handle.net/11467/6123
dc.identifier.urihttps://doi.org/10.1016/j.matpr.2022.07.447
dc.identifier.volume71en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofMaterials Today: Proceedingsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectBiomedical Applications; Electrospun Nanofibers; PCL; Plasmid DNAen_US
dc.titleSynthesis and evaluation of electrospun PCL-plasmid DNA nanofibers for post cancer treatmentsen_US
dc.typeArticleen_US

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