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    Effects of gentamicin-loaded PCL nanofibers on growth of Gram positive and Gram negative bacteria
    (Blue Pen, 2017) Ceylan, Muhammet; Yang, Shang-You; Asmatulu, Ramazan
    Poly-?-caprolactones (PCLs) incorporated with gentamicin of different concentrations (0, 2.5, 5 and 10 wt.%) were electrospun under various conditions, and the resultant nanofibers of different thicknesses (1, 2 and 4 layers) were used against the growth of Gram-negative and Gram-positive bacteria, such as Escherichia coli, Salmonella sp. and Staphylococcus epidermidis. PCL polymer was selected mainly because of its biodegradable aliphatic polyester characteristics and also, it plays a critical role in tissue engineering and pharmaceuticals. Scanning electron microscopy (SEM) images showed that the resultant fibers were in the range of 50 to 200 nm with an average diameter of 100 nm. Bacterial test results revealed that the gentamicin molecules in the nanofibers were gradually released from the PCL nanofibers during the in vitro tests and prevented bacterial growth at different inhibition zones and kinetics. Overall, this work provides a detailed explanation of how to improve the antibacterial properties of new drug delivery systems for many biomedical fields, such as scaffolding; drug, DNA, and protein delivery; and wound healing.
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    Poly-?-caprolactone electrospun nanofiber mesh as a gene delivery tool
    (Aims Press, 2016) Jiang, Jianhao; Ceylan, Muhammet; Zheng, Yi; Yao, Li; Asmatulu, Ramazan; Yang, Shang-You
    Poly-?-caprolactone (PCL) is a biodegradable aliphatic polyester which plays critical roles in tissue engineering, such as scaffolds, drug and protein delivery vehicles. PCL nanofiber meshes fabricated by electrospinning technology have been widely used in recent decade. The objective of this study intends to develop a gene-tethering PCL-nanofiber mesh that can be used as a wrapping material during surgical removal of primary bone tumors, and as a gene delivery tool to provide therapeutic means for tumor recurrence. Non-viral plasmid vector encoding green fluorescent protein (eGFP) was incorporated into PCL nanofibers by electron-spinning technique to form multilayer nano-meshes. Our data demonstrated that PCL nanofiber mesh possessed benign biocompatibility in vitro. More importantly, pCMVb-GFP plasmid-linked electrospun nanofiber mesh successfully released the GFP marker gene and incorporated into the co-cultured fibroblast cells, and consequently expressed the transgene product at transcriptional and translational levels. Further investigation is warranted to characterize the therapeutic influence and long-term safety issue of the PCL nanofiber mesh as a gene delivery tool and therapeutic device in orthopedic oncology